Journal: bioRxiv

Article Title: Prenatal treatment with rapamycin restores enhanced hippocampal mGluR-LTD and mushroom spine size in a Down’s syndrome mouse model

doi: 10.1101/2020.04.08.032029

Figure Lengend Snippet: (A) Hippocampal proteins from WT and Ts1Cje mice pairs were analyzed by Western blots with an anti-FMRP antibody. The signals were normalized to the corresponding total protein loaded and the mean ± SEM values are shown (p = 0.0006, Mann Whitney test, n = 7). (B) Representative Western blot showing two WT and four Ts1Cje littermate pairs (some lanes corresponding to non-littermate animals have been removed). (C) Quantification of the relative amount of FMRP protein in dendrites of WT and Ts1Cje hippocampal neurons at DIV14. The mean pixel intensity ± SEM for FMRP immunofluorescence in dendrites is shown in arbitrary units (a.u.) (p = 0.0006, t test, n = 10). (D) Representative gray scale confocal images from the experiment in panel C, showing FMRP and MAP2 labeling in WT and Ts1Cje neurons. Scale bar = 20 μm. Note that dendritic branching and length are reduced in Ts1Cje neurons, compared to WT controls (unpublished observations).

Article Snippet: We used anti-FMRP (Abcam; Ref. ab69815), anti-MAP2 (Merck Millipore; Ref. MAB378), and anti-LC3B (Cell Signaling; Ref. #2775) as primary antibodies, while horseradish peroxidase (HRP)-conjugated anti-rabbit (Promega), Alexa Fluor 555 goat anti-rabbit (Invitrogen), and Alexa Fluor 488 goat anti-mouse (Invitrogen) were used as secondary antibodies.

Techniques: Western Blot, MANN-WHITNEY, Immunofluorescence, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology

doi: 10.3390/ijms22168922

Figure Lengend Snippet: Alteration of PSD-95 immunostaining within the hippocampal DG in the distinct rat models of PD neuropathology. PSD-95 immunoreactivity within DG of the control rats (Control, C9, C15, C16), the rats with PD cholinopathy (PPT lesion, P1, P9, P10), and the hemiparkinsonian rats with PD cholinopathy (SNpc/PPT lesion, S/P7, S/P8, S/P9). MoDG—molecular layer of the dentate gyrus; GrDG—granule cell layer of the dentate gyrus; PoDG—polymorph cell layer of the dentate gyrus. Scale bar is 25 μm. Whereas PSD-95 immunoreactivity was suppressed in the rats with PD cholinopathy, it was enhanced in the hemiparkinsonian rats with PD cholinopathy within the granular cell and molecular layers of DG vs. the control rats.

Article Snippet: Then, the sections were incubated overnight at +4 °C with the following primary antibodies: mouse monoclonal anti-PV antibody (dil. 1:2000, P3088, Sigma-Aldrich, Burlington, MA, USA) [ ], mouse monoclonal anti-MAP2 antibody (dil. 1:6000, MAB378, Merck Millipore, Burlington, MA, USA), and mouse monoclonal anti-PSD-95 antibody (dil. 1:200, MAB1598, Merck Millipore, Burlington, MA, USA).

Techniques: Immunostaining

Journal: International Journal of Molecular Sciences

Article Title: Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology

doi: 10.3390/ijms22168922

Figure Lengend Snippet: Alteration of PSD-95 immunostaining within the hippocampal CA3 in the distinct rat models of PD neuropathology. PSD-95 immunoreactivity within CA3 of the control rats (Control, C9, C15, C16), the rats with PD cholinopathy (PPT lesion, P1, P9, P10) and the hemiparkinsonian rats with PD cholinopathy (SNpc/PPT lesion, S/P7, S/P8, S/P9). Py—pyramidal cell layer of the hippocampus; Rad—radiatum layer of the hippocampus. Scale bar is 25 μm. PSD-95 immunoreactivity was suppressed in the rats with PD cholinopathy within the pyramidal cell layer and stratum radiatum of the hippocampal CA3 region vs. the control rats and the hemiparkinsonian rats with PD cholinopathy.

Article Snippet: Then, the sections were incubated overnight at +4 °C with the following primary antibodies: mouse monoclonal anti-PV antibody (dil. 1:2000, P3088, Sigma-Aldrich, Burlington, MA, USA) [ ], mouse monoclonal anti-MAP2 antibody (dil. 1:6000, MAB378, Merck Millipore, Burlington, MA, USA), and mouse monoclonal anti-PSD-95 antibody (dil. 1:200, MAB1598, Merck Millipore, Burlington, MA, USA).

Techniques: Immunostaining

Journal: International Journal of Molecular Sciences

Article Title: Hippocampal and Reticulo-Thalamic Parvalbumin Interneurons and Synaptic Re-Organization during Sleep Disorders in the Rat Models of Parkinson’s Disease Neuropathology

doi: 10.3390/ijms22168922

Figure Lengend Snippet: Individual examples of MAP2 and PSD-95 immunostaining alteration within RT in the distinct rat models of PD neuropathology. ( A ) The typical individual examples of MAP2 immunoreactivity within RT in the control rat (Control, C4), rat with PD cholinopathy (PPT lesion, P9), and hemiparkinsonian rat with PD cholinopathy (SNpc/PPT lesion, S/P5). Scale bar is 200 μm. ( B ) The typical individual examples of PSD-95 immunoreactivity within RT in the control rat (Control, C4), rat with PD cholinopathy (PPT lesion, P9), and hemiparkinsonian rat with PD cholinopathy (SNpc/PPT lesion, S/P5). Rt—reticular nucleus; VPM—ventral posteromedial thalamic nucleus; VPL—ventral posterolateral thalamic nucleus; ic— internal capsule. Scale bar is 200 μm. Whereas the partially increased MAP2 expression in RT of the rats with PD cholinopathy was followed by the suppression of PSD-95 expression, the enhanced MAP2 immunoreactivity in RT of the hemiparkinsonian rats with PD cholinopathy was followed by PSD-95 expression similar to control level.

Article Snippet: Then, the sections were incubated overnight at +4 °C with the following primary antibodies: mouse monoclonal anti-PV antibody (dil. 1:2000, P3088, Sigma-Aldrich, Burlington, MA, USA) [ ], mouse monoclonal anti-MAP2 antibody (dil. 1:6000, MAB378, Merck Millipore, Burlington, MA, USA), and mouse monoclonal anti-PSD-95 antibody (dil. 1:200, MAB1598, Merck Millipore, Burlington, MA, USA).

Techniques: Immunostaining, Expressing